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Schematic of the gene targeting strategy and SpCas9 expression induced by Dox. The rtTA is contained within the p Rosa26 locus. The TRE3G-controlled SpCas9-T2A-tdTomato-expressing cassette is contained within the p Hipp11 locus. SpCas9 and tdTomato expression is tightly regulated by Dox. SA, splice acceptor.

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