Gel from Scientific Research

Immunoblot analysis of endogenous (CTCF AID2 ) and induced exogenous (HA-CTCF) expression of CTCF using an antibody for CTCF. CTCF AID2 expression can be seen in all untreated samples (-, -). After 6 h of 10 M 5-Ph-IAA treatment, CTCF AID2 expression is depleted (+, -). Exogenous HA-CTCF expression comparable to endogenous CTCF was only observed following 18 h of 1 g/mL doxycycline (+, +).

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