Illustration from Scientific Research

Design of the fusion guide RNA construct of CCRS. The CasRx guide RNA has a 5'-scaffold sequence in front of the antisense RNA sequence, and the ribozyme shares the same 5'-antisense RNA with crRNA. The fusion guide RNA has scaffolds for CasRx and ribozyme, enabling RNA cleavage with both CasRx and ribozyme. H at position 7 designates A, C, or U. N indicates any nucleotide.

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