Extracellular amino acids and uracil levels, measured by targeted metabolomics38(seeSTAR Methods), in wild-type and SeMeCo cultures during CLS. Bar plots show mean ± SEM FC to mean wild-type levels in the exponential phase of n = 4 biological replicates/strain. The shared essential metabolites in SeMeCoH, L, U, and Mare highlighted in bold. Insets boxplots represent median (50% quantile, middle line) and lower and upper quantiles (lower [25% quantile] and upper [75% quantile]) of pooled metabolite levels of 4 biological replicates/strain/growth phase; each dot represents a metabolite in a biological replicate. Adjusted p values inTable S1. Statistics by unpaired two-sided t test (C) and Wilcoxon rank-sum test (D) and multiple testing correction using the BH method. p value∗p < 0.05,∗∗p < 0.005,∗∗∗p < 0.0005, and∗∗∗∗p < 0.00005. See alsoFigure S2.
