(i) Prototrophic wild-type yeast cells supplemented either with12C- or13C-glucose, followed by media swap for isotope tracing amino acid analysis, using targeted metabolomics38(seeSTAR Methods). (ii) Fractions of13C-labeled amino acids in cultures initially grown in12C-glucose and swapped to13C-glucose, at different time points post media swap, as described in (i). Bar plots show mean ± SEM of 4 biological replicates (individual dots represent independent cultures); individual fraction values and p values inTable S2. Statistics for (B), (C), and (E) by unpaired two-sided Wilcoxon rank-sum test and multiple testing correction using the Benjamini-Hochberg (BH) method. See alsoFigure S1.
