Targeted metabolome analysis for the quantification of intracellular nucleotide, amino acid, glycolytic, and tricarboxylic acid (TCA) cycle metabolites in wild-type yeast cells cultured in synthetic minimal (SM) medium at E, ES, and S phases, as described in (A). (i) Principal-component analysis (PCA); individual dots represent independent cultures. (ii) Intracellular metabolite concentrations shown as fold change (FC) to the exponential phase according to metabolite classes and growth phases. Boxplots represent median (50% quantile, middle line) and lower and upper quantiles (lower [25% quantile] and upper [75% quantile], respectively) of pooled metabolite levels of n = 4 biological replicates. Each dot represents a metabolite in a biological replicate. Adjusted p values*p < 0.05,**p < 0.005,***p < 0.0005, and****p < 0.00005; adjusted p values inTable S1.
