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B-cell uptake and activation was measured by flow cytometry on dLNs on day 1 and 3 (n = 4 and 4 mice for TLR7–alum, n = 4 and 4 mice for TLR7-NP). Data in d – f are mean ± s.e.m. All the data are analysed by two-sided, unpaired t -test with Welch’s correction. P values are as shown. Data were combined from two independent experiments ( b , c , d , f ) or from one representative of two independent experiments ( a , e ).
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