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HPLC analysis of the enzymatic conversion of 7: enzyme-free mixture as a control (i) and reactions conducted in the presence of PyrI4 (ii), PloI4 (iii) and PloI4 in the dark (iv). In a – d, the parts of the structures and HPLC peaks associated with the different cycloaddition reactions are indicated by colour: green, exo -4 + 2; blue, endo -4 + 2; yellow, exo -2 + 2.
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