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Indicated mutants of lipase-like proteins (SETI_wt, SETI_sag101, SETI_pad4, SETI_pad4sag101, and SETI_eds1) were sown on round germination medium (GM) plates with or without E2 (50 M), and their growth arrest phenotype was recorded 18 days after planting on E2 plates. (Note: we have used the full name of all the mutants in all the figures for better visualization.)
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