Gel from Scientific Research

Genomic PCR to detect NIA1 edits in the wild-type scion progeny. Edited NIA1 PCR amplicons are marked by stars. All samples were harvested from 10-day-old seedlings grown on NH 4 -limited plates. Each replicate is a pool of ~70 to ~100 seedlings (Supplementary Table 1 ). Cas9 and Kan transcript RTqPCRs were used to confirm that the progeny was transgene-free.

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