Bar Plot from Scientific Research

Alanine scanning of Trp2 peptide using mutant Trp2 peptides individually pulsed on EL4 cells and incubated with 1 g ml 1 scFv clone 13. scFv13 bound levels were determined by 6-His-tag FITC antibody staining. Representative percentages of FITC-positive cells are shown as mean s.d. (n = 3 biological triplicates). Upward-, downward- and sideways-facing amino acids are indicated by red, green and blue arrows, respectively. The modeled Trp2 9mer peptide is shown as a magenta stick, with each position indicated.

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