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Left: expression patterns of genes that show significant changes (q< 0.05) along pseudotime. Zscores of expression levels during the pseudotime were calculated for the detected genes. The detected genes were classified into ten gene clusters (GC1 to GC10) by k-means clustering (k = 10). The horizontal axis is pseudotime (from protonema to gametophore, left to right). Numbers at the top of the heatmap correspond to the UMAP plot’s subcluster number shown in (D). Dashed gray lines indicate gametophore apical cells enriched pseudotime, the earliest region of the pseudotime in subcluster 4. Right: significant GO terms enriched in each GC. Only “driving terms” detected by g:profiler software are shown to avoid redundancy. See also Figure S3 and Table S1.
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