Workflow from Scientific Research

Chem-map workflow—in permeabilized cells, a precomplex of biotinylated small molecules (yellow) and antibiotin antibody (black) bind to the chromatin target (protein or DNA). Then a secondary antibody (purple) tethering of pA–Tn5 transposomes (light blue) was recruited to the drug binding sites. Addition of Mg 2+ activates the transposomes and integrates adapters (green and orange) in the proximity of the drug-binding sites. After DNA purification, genomic fragments with adapters at both ends are enriched via PCR, which allows the genome-wide identification of drug-binding sites by next-generation sequencing.

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