Real-time fluorescence signal-navigated cryo-FIB milling by ELI-TriScope. During the cryo-FIB process, the cryo-fluorescence intensity of the target is monitored in real time. In the SEM and fluorescence images from left to right, three different stages during cryo-FIB milling are presented: the beginning stage (left), the middle stage with one side rough milled (middle) and the final stage with two sides fine milled (right). The fluorescence signals from two centrioles are indicated by red and orange arrows and monitored in real time. A line profile of the intensity of the fluorescence signal is correspondingly plotted below for the three stages. A schematic diagram of the thickness of the cryo-lamella at different stages is shown at the bottom.
