Microscopy from Scientific Research

Enlarged rendering of HyU results (e, white box) clearly shows low levels of bleed-through between labels (m–p). Similar enlargement of LU results show noticeably worse performance. Note that regions with bright signals (membrane j, n, white arrow) bleed through other channels (m, o). Scale bar, 20 m. Tetra-labeled specimen used here was Gt(cltca-citrine);Tg(ubiq:lyn-tdTomato; ubiq:Lifeact-mRuby;fli1:mKO2). The sample depicted in e is representative of 28 experimental sessions each with three to five biological replicates, yielding similar results.

DOI