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Diagram illustrating the isolation of the R 2 lines. Double green (G-/G-) and double red (-R/-R) seeds were selected from the progeny of sequenced recombinants and crossed (F 1 ). Recombinant GR/-R F 2 seeds were visually selected and backcrossed to wild-type or msh2 plants; at each generation, GR/-- seeds were selected. The BC 3 plants were used to measure ChP CO frequency.
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