Quantification of the fluorescence intensity of H3K27me3 staining at the nuclear envelope at different stages of differentiation (Prog, progenitors; Diff, differentiating; Multi, multiciliated differentiated cells) on images as in (D) normalized to the mean of intensity in progenitors of the same image. Error bar represent the SEM of n= 407, 111, and 275 cells for Prog, Diff, and Multi cells, respectively, in H3K27me3 staining andn= 681, 143, and 338 cells for Prog, Diff, and Multi, respectively, in H3K9me2 staining in three independent experiments each.***p ≤0.001;**p< 0.01 (one-way ANOVA followed by Dunn’s multiple comparison).
