Bar Plot from Scientific Research

mRNA levels of Nrg1 in low glucose (LG)- or high glucose (HG)-treated Eo771 cells lacking parts of the Nrg1 enhancer elements, as indicated. Data represented mean SEM of technical replicates in a, and biological replicates in c, e, f, h, j, and n = 3 independent experiments were performed in a-h, n = 1 for i, j. Statistical significance was evaluated using two-way ANOVA followed by Tukey’s a, j and Bonferroni’s c post hoc test, and one-way ANOVA followed by Tukey’s post hoc test e, and two-tailed Student’s t -test f, h. * P < 0.05; ** P < 0.01; *** P < 0.001 for column 1 versus others, and # P < 0.05; ## P < 0.01; ### P < 0.001 for column 2 versus column 4, a (from left to right, *** P < 0.0001, *** P < 0.0001, ** P = 0.0024, *** P = 0.001, *** P < 0.0001), c (from left to right, * P = 0.0277, * P = 0.042, * P = 0.0166, * P = 0.0182, P = 0.0556), e (from left to right, ** P = 0.0011, *** P = 0.0005), f (*** P < 0.0001), h (** P = 0.0049), j (from left to right, * P = 0.0487, ** P = 0.008, ** P = 0.0099, # P = 0.0486). Source data are provided in Source Data file.

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