Sequential ChIP-qPCR analysis of binding co-occupancy of RBPJ with P300, CBP, and SETD1A within the Nrg1 enhancer region in Met1 cancer cells treated with LG or HG. Data represented mean ± SEM of technical replicates in a–j , and n = 3 independent experiments were performed. Statistical significance was evaluated using two-way ANOVA followed by Bonferroni’s post hoc test a – j . * P < 0.05; ** P < 0.01; *** P < 0.001, a (all *** P < 0.0001), b ( *** P < 0.0001), c (from left to right, * P = 0.0133, ** P = 0.0013, *** P < 0.0001), d (from left to right, * P = 0.0234, *** P < 0.0001, * P = 0.0378, *** P = 0.0006), e (from left to right, *** P < 0.0001, * P = 0.0403, ** P = 0.0049, *** P < 0.0001), f (from left to right, ** P = 0.0019, ** P = 0.0016, *** P < 0.0001), g (from left to right, *** P = 0.0003, * P = 0.0323), h (from left to right, ** P = 0.0066, * P = 0.0349, *** P < 0.0001), i (from left to right, * P = 0.033, *** P = 0.0005, ** P = 0.0047), j (from left to right, * P = 0.0279, *** P < 0.0001, * P = 0.0126). Source data are provided in Source Data file.
