ChIP-qPCR analysis of binding occupancy of RBPJ, NOTCH intracellular domain (NICD), P300, CBP, SETD1A, HDAC and enrichment of H3K4me1 and H3K27ac within the Nrg1 enhancer region in Met1 cancer cells treated with vehicle or DAPT under HG. Data represented mean ± SEM of technical replicates in a–j , and n = 3 independent experiments were performed. Statistical significance was evaluated using two-way ANOVA followed by Bonferroni’s post hoc test a – j . * P < 0.05; ** P < 0.01; *** P < 0.001, a (all *** P < 0.0001), b ( *** P < 0.0001), c (from left to right, * P = 0.0133, ** P = 0.0013, *** P < 0.0001), d (from left to right, * P = 0.0234, *** P < 0.0001, * P = 0.0378, *** P = 0.0006), e (from left to right, *** P < 0.0001, * P = 0.0403, ** P = 0.0049, *** P < 0.0001), f (from left to right, ** P = 0.0019, ** P = 0.0016, *** P < 0.0001), g (from left to right, *** P = 0.0003, * P = 0.0323), h (from left to right, ** P = 0.0066, * P = 0.0349, *** P < 0.0001), i (from left to right, * P = 0.033, *** P = 0.0005, ** P = 0.0047), j (from left to right, * P = 0.0279, *** P < 0.0001, * P = 0.0126). Source data are provided in Source Data file.
