Centriolar γ-tubulin intensities were quantified in cells as in ( c ), normalized to the average of the intensities of the control, and plotted. N = 4 experiments, total number of cells analyzed for control and ch-TOG RNAi, respectively: 116 and 116 (-); 120 and 117 (ch-TOG), 122 and 120 (ch-TOG-12345), 112 and 113 (ch-TOG-C), 113 and 95 (ch-TOG-345C). *** p = 0.0002 (-), p = 0.4899, not significant (ch-TOG), **** p < 0.0001 (ch-TOG-12345), **** p < 0.0001 (ch-TOG-C), *** p = 0.0005 (ch-TOG-345C). The horizontal bars and whiskers indicate median and interquartile range, respectively, of the plotted data points. Statistical significance was determined by unpaired, two-tailed t test with Welch’s correction.
