Cells as in ( c ) were subjected to mi c rotubule depolymerization by incubation on ice at 4 °C for 30 min. Following microtubule regrowth for 5 s at 37 °C, α-tubulin intensities of microtubule asters around centrosomes were quantified, normalized to the average of the intensities of the control, and plotted. N = 2 experiments, total number of cells analyzed for control and ch-TOG RNAi, respectively: 49 and 50 (-); 48 and 47 (ch-TOG), 50 and 48 (ch-TOG-12345), 49 and 50 (ch-TOG-C), 50 and 49 (ch-TOG-345C). ** p = 0.0084 (-), p = 0.3937, not significant (ch-TOG), * p = 0.0169 (ch-TOG-12345), ** p = 0.0062 (ch-TOG-C), * p = 0.0136 (ch-TOG-345C). The horizontal bars and whiskers indicate median and interquartile range, respectively, of the plotted data points. Statistical significance was determined by unpaired, two-tailed t test with Welch’s correction. Source data are provided as a Source Data file.
