Intensities of centriolar gamma-tubulin, NEDD1, and PCNT staining in cells as in ( a ) were quantified, normalized to the average of the respective controls, and plotted. gamma-tubulin staining: N = 3 independent experiments; total number of cells analyzed, 75 (Control RNAi) and 69 (ch-TOG RNAi); *** p = 0.0004. NEDD1 staining: N = 3 independent experiments; total number of cells analyzed, 312 (Control RNAi) and 327 (ch-TOG RNAi); ** p = 0.0042. PCNT staining: N = 2 independent experiments; total number of cells analyzed, 151 (Control RNAi) and 129 (ch-TOG RNAi); p = 0.3865 (ns, not significant). The horizontal bars and whiskers indicate median and interquartile range, respectively, of the plotted data points. Statistical significance was determined by unpaired, two-tailed t test with Welch’s correction without multiple comparison.
