Gel from Scientific Research

Lysates from HEK293T cells transiently expressing Flag-BirA or Flag-BirA-GCP3 and grown in the presence of biotin for 24 h were subjected to affinity pulldowns using anti-FLAG antibody and streptavidin-coupled beads. The pulldown precipitates were subjected to immunoblot and probed with anti-ch-TOG, anti-gamma-Tubulin, anti-FLAG, and anti-GAPDH antibodies. Detection of GAPDH was used as control. The results were replicated in two independent experiments. Source data are provided as a Source Data file.

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