Intensities of endogenous ch-TOG staining at the PCM and at subdistal appendages in cells with or without microtubules as in ( a ) were quantified, normalized to the average intensities in cells with microtubules, and plotted. N = 3 independent experiments. Total number of cells analyzed for PCM staining: 55 (MTs+) and 56 (MTs−). * p = 0.0368. Total number of cells analyzed for subdistal appendage staining: 55(MTs+) and 56 (MTs−). * p = 0.0142. The horizontal bars and whiskers indicate median and interquartile range, respectively, of the plotted data points. Statistical significance was determined by unpaired, two-tailed t test with Welch’s correction.
