Centriolar fluorescence intensities of the indicated proteins in cells as in ( d ) normalized to the average of the control were quantified and plotted. Results are from N =2 independent experiments, total number of cells analyzed: 48 in control and CEP128 RNAi, ** p =0.0028 (ch-TOG staining); 30 in control and 29 in ch-TOG RNAi, p =0.4051 (CEP128 staining); 77 in control and 74 in ch-TOG RNAi, p =0.2148 (ODF2 staining). Statistical significance was determined by unpaired, two-tailed t test with Welch’s correction. Ns, not significant. The horizontal bars and whiskers indicate median and interquartile range, respectively, of the plotted data points. Illustrations indicate centriole orientations in the respective images. Scale bars, 1 m. Source data are provided as a Source Data file.
