Microscopy from Scientific Research

Open access visualization of Microscopy, TJ formation, CTRLsh hiPSCs, GPC4sh hiPSCs, Cell dissociation
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DOI: 10.1038/s41467-023-35965-8

DOI

Time course analysis of TJ formation in CTRLsh and GPC4sh hiPSCs after cell dissociation and seeding in combination with the analysis of pSMAD1,5 expression in response to BMP4 stimulation for 2, 6, 9, 12 and 18 h. Cells were subjected to immunofluorescence analysis of pSMAD1,5 (magenta) and ZO1 (green) proteins, n = 3. Scale bar: 100 m.

#Microscopy#TJ formation#CTRLsh hiPSCs#GPC4sh hiPSCs#Cell dissociation#Cell seeding#pSMAD1,5 expression#BMP4 stimulation#Immunofluorescence analysis#pSMAD1,5 protein#ZO1 protein

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