Quantitative analysis from the staining shown in a of pSMAD1,5 positive cells (left panel) and of ZO1 organized areas (middle panel) in CTRLsh and in shGPC4 hiPSC cultures. Note the different kinetics in GPC4sh cultures versus controls. The right panel show the combination of the left and middle panels to highlight the correlation between the percentage of pSMAD1,5 positive cells and of ZO1 organized area. Data are represented as mean SEM, n = 3 for pSMAD1,5 staining and n = 4 for ZO1 staining. Statistical analysis: Statistical analysis: ( b ) two-way ANOVA followed by Sidak’s multiple comparison test were used to analyzed pSMAD1,5 counts and unpaired t -test were used to analyzed ZO1 organized areas P -values: (***) <0.001, (*) <0.05. For all panels “ n ” corresponds to the number of biological replicates unless stated otherwise. Source data are provided as a Source Data file.
