Analysis of LEF1 normalized intensity in organized and disrupted areas of GPC4sh5-c10 029 hiPSCs from staining shown in d, represented as a box and whisker plot; whiskers represent 1st and 99th percentiles, n = 3 with 7957 to 26429 single nucleus values per condition. Statistical analysis for the overall figure: (b) two-way ANOVA followed by Dunnett’s multiple comparison test, (c) linear regression analysis, (e) two-way ANOVA followed by Sidak’s multiple comparison test. P -values: (***) <0.001, (**) <0.01, (*) <0.05, ns not significant. For all panels “n” corresponds to the number of biological replicates unless stated otherwise. Source data are provided as a Source Data file.