Percentage of BRACH positive cells as a function of distance from the zone with disrupted TJs were quantified from staining shown in e . 0 m indicates cells located within the zones of disrupted TJs, +30 m, +60 m, +90 m and >+90 m indicate cells located at 0–30 m, 30–60 m, 60–90 m or >90 m away from zones of disrupted TJs, respectively. Slope of the curves are represented as m . Data are represented as mean SD. n = 3. Statistical analysis for the overall figure: ( d ) two-way ANOVA, followed by Dunnett’s multiple comparison test, ( f ) linear regression analysis. P -values: (***) <0.001, (**) <0.01, (*) <0.05, ns not significant. For all panels “ n ” corresponds to the number of biological replicates unless stated otherwise. Source data are provided as a Source Data file.
