Percentages of BRACH, SOX17 and PGFDRa positive cells in WT, CTRLsh, GPC4sh5-c10 and GPC4sh2-c3 029 hiPSCs were quantified from staining shown in f . Box plots represent the median with min and max values, n = 3. Statistical analysis for the overall figure: ( d , e ) two-way ANOVA followed by Sidak’s multiple comparison test, ( g ) two-way ANOVA, followed by Dunnett’s multiple comparison test. P -values: (***) <0.001, (**) <0.01, (*) <0.05, ns not significant. For all panels “ n ” corresponds to the number of biological replicates unless stated otherwise. Source data are provided as a Source Data file.
