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Total protein extract of WT, CTRLsh, GPC4sh5-c10 and GPC4sh2-c3 029 hiPSCs at the undifferentiated stage were analyzed by Western-blot with pAKT S473, pAKT T308, AKT, pERK T202/Y204, ERK, pGSK3a, b S9/S21, GSK3a, b, pSMAD2 S465/S467 and SMAD2 antibodies. Note similar expression and phosphorylation levels of AKT, ERK, GSK3a, b and SMAD2 proteins in all hiPSC lines. ACTIN protein levels were used as loading control, n = 3. Blots were processed in parallel.

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