Bar Plot from Scientific Research

PCR amplicons from the target regions were analyzed by targeted deep sequencing. The reads harboring only correct edits were counted to evaluate the editing efficiency, and the reads harboring any undesired insertion or deletion were counted to evaluate the indel frequency. The gray bar indicates the indel frequency coupled with the editing efficiency indicated by the left closest bar. Statistical analysis of the normalized increases in intended editing efficiencies. Bars represent the mean of n = 3 independent replicates. The error bars in all figures represent the SDs. The P values were calculated by a two-tailed t test. *** P < 0.005; ** P < 0.01; * P < 0.05.

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