Bar Plot from Scientific Research

Editing efficiency of 2*GCN4-PE2 and 3*GCN4-PE2. Two or three copies of the GCN4 peptides were fused to the N-terminus of the nCas9 enzyme, and the resulting constructs were named 2*GCN4-PE2 and 3*GCN4-PE2, respectively. At the two genomic loci of HEK3 and FANCF, the editing efficiencies induced by prime editors with the increased copy number of GCN4 were determined. The gray bar indicates the indel frequency coupled with the editing efficiency indicated by the left closest bar. Bars represent the mean of n = 3 independent replicates. The error bars in all figures represent the SDs.

DOI