Box and whisker plots showing the median selection coefficients of groups of mutants with specific substitutions at the indicated positions. Color gradient from blue ( hcm1-3N8A ) to red ( hcm1-3N8E ). Blue corresponds to all mutants with A-P at the indicated site, red corresponds to all mutants with E-E at the indicated site. The black center line indicates the median, boxes indicate the 25th−75th percentiles, black lines represent 1.5 IQR of the 25th and 75th percentile, black circles represent outliers. Shown is all data from n = 3 biological replicates. Supplementary Fig. 3b shows correlations between replicates; Supplementary Fig. 5 shows elevated expression of Hcm1-3N and heatmaps comparing A/E and 3N A/E screens.
