Doubling times of strains from ( b ) growing at 30 C in rich medium with 5 g/mL nocodazole. Gray represents WT, color gradient from blue ( hcm1-8A ) to red ( hcm1-8E ) corresponds to increasing numbers of phosphomimetic mutations. Error bars represent standard deviation, shown is an average of n = 3 replicates. Significance was tested using repeated measures one-way ANOVA with Dunnett’s multiple comparisons test. Asterisks indicate significantly different from WT, **** P < 0.0001, *** P < 0.0005, ns = non-significant. d AAAAAAAA P < 0.0001; AAAEAAAA P = 0.0002; AAAAEAAA P = 0.0003 ; AAAEEAAA P = 0.1666; EEEAAEEE P = 0.7932; EEEAEEEE P = 0.9971; EEEEAEEE P = 0.9997; EEEEEEEE P = 0.9886.
