DLS measurements of vesicles after GUV sonication. Before sonication, anionic GUVs were either incubated with TFAMoplexes containing TFAM-A4 or left untreated. CaCl2 was added in all conditions. The sonicated samples were centrifuged, and the supernatants were analyzed by DLS. The experiment was performed independently three times, with mean values of technical triplicates displayed as curves. The embedded legend corresponds to (A) and (B).