Workflow from Scientific Research

Experimental design. (Top left) Animal positioned on a spherical treadmill, capable of running or grooming, while L5 cortical neuron activity is recorded in 2P microscopy using GCaMP8s, and D1R is optically stimulated with MP-D1ago24 in the striatum. (Bottom left) Schematic of the experimental timeline, showing P-D1ago24 infusion, calibration, and the timing of optical stimulation. Blue represents blue light photo-activation, and violet indicates UV photo-deactivation, administered at variable intervals based on the specific experiment. (Center top) Image of GCaMP activity recording, highlighting four directly controlling neurons labeled either E1 or E2. (Center bottom) An auditory tone paired with neural cursor values is provided as feedback to the mouse. (Right) Decoder where the neural cursor is derived from the difference between the E2 and E1 calcium signals. T1 and T2 denote predefined thresholds for this neural cursor. T1 is paired with D1R activation, whereas T2 is not. The X = x axis represents time; the y axis represents the amplitude of calcium transients

DOI