The bar chart (I) and heatmap (J) illustrate the comparison between the traditional qPCR method and our current circular crRNA-assisted photocontrollable one-pot nucleic acid detection method in detecting EBV-infected blood samples in clinical settings. N.D., not detected. The red dashed line in (I) denotes the clinically established threshold of 500 copies/mL for EBV-DNA viral load. Values exceeding this threshold are indicative of EBV positivity in clinical diagnostics, as supported by the current literature and diagnostic standards.52,53This demarcation serves as a critical reference for interpreting EBV infection status in both experimental and clinical contexts. In all panels, error bars represent the mean ± standard deviation (SD) (n= 3).p> 0.05 indicates no significant difference between groups.
