Scatter Plot from Scientific Research

Contour plots represent the flow cytometry analysis of the original library and fluorescence-activated cell-sorted outputs (sorts 1–4) treated with matriptase and labeled with both cleavage and expression reporters. Thexaxis corresponds to the signal from the expression reporter (ABD domain), indicating cell surface display levels, while theyaxis corresponds to the signal from the cleavage reporter (affibody domain), representing whether the substrate has been cleaved. The sorting was performed by gating the cells located in the lower right quadrant. A representative gate is shown in the plots.

DOI