Histograms of relatedness score estimations by deepKin on unrelated individual pairs using different numbers of markers (m= 1,000, 5,000, 10,000, and 50,000). Only unrelated individuals (n= 2,000) are simulated. The line in black indicates the deepest significant relatedness calculated at the significant level of = 0.05/1,999,000. Lines in color indicate KINGs lower boundaries of 0.707 (blue), 0.354 (yellow), 0.177 (green), 0.088 (red), and 0.044 (purple) for zero, first, second, third, and fourth degree of relatedness. Lower boundaries that are smaller than deepest relatedness supported by deepKin are plotted as dashed lines. The gray background indicates the observed relatedness range.