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Schematic overview of the screening setup used for the CRISPRgenee screen. A library composed of 3,686 sgRNAs targeting 1,137 chromatin-related genes was virally transduced into TF-1 erythroleukemia cells expressing ZIM3-Cas9. After antibiotic selection, cells were treated with Dox for 14 days.
#Workflow#Pie Chart#Illustration#CRISPR Screen#sgRNAs#Chromatin-Related Genes#Erythroleukemia Cells#Antibiotic Selection
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