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EMT was induced in MCF10A cells expressing either dCas9-ZIM3 or ZIM3-Cas9 and scr (ctrl) or SMAD2-targeting sgRNAs using TGFβ. The fold change of cells detected in the epithelial and mesenchymal population was calculated in SMAD2-depleted cells relative to the control (n= 3, mean ± SEM).

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