Box Plot from Scientific Research

Time-resolved quantification of CD13 and CD33 negative TF-1 cells expressing the indicated sgRNAs after induction of ZIM3-Cas9. A total of eight CRISPRko (ko) and seven CRISPRi (i) sgRNA designs were combined in 22 CRISPRgenee (g) constructs for comparison. Data are displayed as a single datapoint for each sgRNA or sgRNA combination and replicate summarized in a boxplot (n= 3, mean, box, and whiskers minimum to maximum); ∗p≤ 0.05, ∗∗p≤ 0.01, ∗∗∗p≤ 0.001; n.s., non-significant. Significance was determined using a one-way ANOVA for (D) and a two-way ANOVA for (B), (C), and (E). See alsoFigures S1andS2.

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