Top: time courses of Ca2+current densities after whole-cell break-in of Orai1-C143X-V181Azi (B)/Bpa (C) and Orai1-C143X-A177Azi (D) (X = A, C, L) mutants. The application of UV light (15 s; purple lightning unless indicated by the colored bar in B and C) triggered UV-induced activation or no change of Ca2+currents. Bottom: correlation for mutants Orai1-C143X (X = G, A, S, C, N, L, F, W)-V181Azi (B)/Bpa (C) and Orai1-C143X (X = A, S, C, L, F)-A177Azi (D) between current density and the side-chain volume of the substitution at position 143 before (black dots) and after (red dots) UV light. All data (B-D, G, and H) represent mean values ± SEM of indicated number (n) of experiments.
