Differentially enriched proteins highlighted in blue (two-sample t test, FDR <5%). Illustration of signaling pathways affected and the proposed role of REPS1 in vesicle trafficking. The illustration was made in BioRender. *symbol refers to significance of inhibitor vs. DMSO treatment. # symbol refers to significance of insulin/contraction. ***/###p< 0.001, **/##p< 0.01, */#p< 0.05. Data in (A), (B), (C), and (D) were analyzed by two-way ANOVA with Tukey’s multiple comparisons test. Data in (E)–(G) and (I)–(K) were analyzed by a two-way ANOVA with repeated measures with idák’s multiple comparisons test (n= 7–8). In paired analyses, bars represent the mean. In unpaired analyses, the mean +/- standard error of the mean is displayed.
