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Representative IHC images and quantification of granzyme B in Colon26 tumor sections. The images are representative of three mice, with ~5 fields of view per sample. Scale bars, 50 μm. G1, PBS; G3, EcN/CR; G4, EcN/CR + laser; G5, EcN-IL-15/CR; G6, EcN-IL-15/CR + laser. Data are presented as means ± SEM.pvalues were analyzed by two-tailed unpaired Student’s t test (D, F, G) or one-way ANOVA with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli test (C, E), Holm-Sidak’s multiple comparisons test (K). *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.0001; ns, not significant.
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