Volcano plots showing detected proteins by quantitative proteomics after treatment with CPT (100 nM), Chk1i (100 nM), AcD (100 nM), or BMH-21 (10 M) for 6 h. The threshold for downregulation (<0.66-fold change), upregulation (>1.5-fold change), and statistically significant changes (p< 0.01) are marked with dotted lines. The number of downregulated (blue) and upregulated (red) proteins is shown in parentheses, with key proteins annotated. p values were calculated using one-way ANOVA + t test with Bonferroni correction. Data are from biological triplicates for CPT/control and Chk1i/control and technical triplicates for AcD/control and BMH-21/control.
