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Quantification of nascent or total RNA for indicated genes using real-time qPCR in cells treated with 100 nM CPT. The y axis shows fold changes in nascent or total RNA levels normalized to the total 18S ribosomal RNA between treated and untreated samples. Data are presented as mean ± SD from four or five independent experiments.*p< 0.05;**p< 0.01; n.s., not statistically significant (unpaired two-tailed t test).
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