Microscopy from Scientific Research
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Time-lapse images of Dendra2-occludin before (green) and after irreversible photoconversion (red) at TJs in BEC monolayers grown on collagen or Ncdh-BioS. Enlarged images on the right: recruitment (green, = 488) and internalization (red, = 543) of occludin within the irradiation region at TJs (dashed squares) at different times before and after photoconversion at t = 0:00. Dashed lines indicated photoconverted area. Scale bar, 20 m; inset, 3 m; time in minutes.
#Microscopy#Time-Lapse Imaging#Dendra2-Occludin#Photoconversion#Tight Junctions#Brain Endothelial Cells#Collagen#N-Cadherin Biomimetic Surfaces#Occludin Recruitment#Occludin Internalization
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